High Quality of Ponceau S CAS 6226-79-5 C. I. 27195 4-Sulfophenylazo

• CAS No.: 6226-79-5
• Formula: C22h12n4na4o13s4
• EINECS: 209-322-8
• Classification: Organic Analytical Reagents
• Grade: AR
• Specific Usage: For Synthesis
• Model NO.: SUREST-119
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
• Source: Dry Powder
• Habit Appellation: Special Reagent
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture
• Property: Organic Reagent
• Shelf Life: 2 Years
• Stock: Available
• Molecular Weight: 760.57
• Color: Bordeaux Red
• Transport Package: 25kg /Drum
• Specification: 98%min
• Trademark: Surest
• Origin: China
• HS Code: 3204901000
• Production Capacity: 1000 Tons Yearly

Product Description

Ponceau S
 

Name	Ponceau S
Synonyms	C.I. 27195; 3-Hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic acid tetrasodium salt
Molecular Structure
Molecular Formula	C22H12N4Na4O13S4
Molecular Weight	760.57
CAS Registry Number	6226-79-5
EINECS	228-319-2

Properties

Water solubility	10 g/L

 

Chemical Property/Description

Bordeaux red to dark red or brown powder,Ponceau S is negatively charged and can bind to positively charged amino acid residues, while ponceau can also bind to non-polar regions of proteins to form red bands. The staining of proteins by Ponceau S is reversible and can be washed away with distilled water, PBS, or other appropriate solutions.

Main Uses:

Biological staining, protein staining after electrophoresis. Ponceau S can be used to detect proteins attached to NC and PVDF membranes, but cannot be used for Nylon membranes.

Determination of Cu in food by ponceau S probe dual wavelength absorption spectrometry. In TRIS-hydrochloric acid buffer medium at pH 6.86, ponceumin S reacts with Cu(2) to form an ion association with an obvious positive absorption peak and a clear negative absorption peak in the visible region. The positive and negative absorption wavelengths are 445 nm and 510 nm, respectively. Cu(2) follows Lambert-Bier's law in 0.06~1.6 mg/L(positive absorption, 445 nm), 0.04~1.6 mg/L(negative absorption, 510 nm) and 0.03~1.6 mg/L(DWO-VIS, 445 nm+510 nm). The apparent molar absorptivities (κ) were 8.70× 10-3 (positive absorption), 2.04× 10-4 (negative absorption) and 2.92× 10-4 (DWO-VIS)L/(mol·cm), respectively. The limits of quantitation were 0.62, 0.38 and 0.26 mg/100 g, respectively. The effects of absorption spectra, suitable reaction conditions and co-existing substances were also discussed. The dual-wavelength method was used to determine Cu in biscuits, noodles and milk powder. The recoveries were 97.9%-102% and the relative standard deviations (RSD) were 2.5%-2.8% (n=5).